412 Development of Bead-Based Multiplex Immunoassay for Antimicrobial Peptide Detection in Stratum Corneum of Atopic Dermatitis Skin

Atopic dermatitis (AD) is a heterogeneous disease and characterized by dysregulated immune response of diverse cell subtypes, such as Th2, Th22, Th17. Heterogeneity AD can be reasoned varying contribution underlying subsets, observed altered cytokine milieus in the skin. These variations result changes protein expression keratinocytes, including antimicrobial peptides (AMPs). We hypothesize that peptide may correlate with subtypes and, therefore, used potential biomarkers to predict treatment outcome. In this study, we non-invasive tape stripping collect stratum corneum samples determine concentration AMPs on skin patients under systemic or topical treatment. To quantify AMP developed new magnetic bead-based multiplex immunoassay, allowing simultaneous detection six different (HBD-2, HBD-3, RNase 7, Dermcidin, S100A7 S100A8/S100A9 Heterodimer). found differs lesional compared healthy controls, depending current efficacy body site. Observed point towards subsets. Therefore, our assay represents useful tool for